Carotenoids are important pigments, due to their antioxidant activity. The purpose of this research was to observe the effect of GYEA media with FeSO4 and FeCl2 on the total carotenoid content (TCC) and the carotenoid profile of Rhodococcus sp. and Gordonia sp. In the biomass of Rhodococcus sp., with 0.025% FeSO4 a TCC of 14.91±1.66 µg/g was calculated, and two carotenoids were observed; the Carotenoid 2, identified as an astaxanthin dirhamnoside like carotenoid increased from 13.45±0.52% in GYEA media without salts to 24.21±1.48% with 0.025% FeSO4 (T value of 11.81, α 0.05). With the biomass of Gordonia sp. in the presence of 0.025% FeCl2 a TCC of 28.66±8.21 µg/g was calculated and three carotenoids were observed, no significant increase was determined for these carotenoids. The formation of carotenoid-iron complexes was observed with UV-Vis.